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The Effect of Splenectomy on the Recruitment of Inflammatory Cells in the Cerebral Microvasculature
Author(s) -
Seifert Hilary,
Russell Janice,
Granger Daniel Neil
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.723.6
Subject(s) - splenectomy , spleen , medicine , inflammation , platelet , systemic inflammation , intravital microscopy , lipopolysaccharide , pathology , stroke (engine) , immunology , microcirculation , mechanical engineering , engineering
Splenectomy decreases inflammatory cell recruitment to the brain following sterile cerebral inflammation that results from stroke. Similarly it is known that non‐sterile cerebral inflammation like systemic lipopolysaccharide (LPS) causes rapid recruitment of leukocytes in the cerebral venules. It is not known if the protection afforded by splenectomy is specific to stroke or if removal of the spleen can protect the brain against systemic LPS. The objective of this study was to determine if splenectomy alters the recruitment of adherent leukocytes and platelets in the cerebral microvasculature following systemic LPS administration. C57Bl/6 mice underwent either splenectomy or sham splenectomy two weeks prior to an intra‐peritoneal injection of LPS (10 ug/mouse). Four hours after LPS injection, mice were imaged with intravital video microscopy. Adherent platelets, labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE), and leukocytes, labeled with rhodamine G6, were quantified. Peripheral blood counts were also measured in all groups. LPS significantly increased the number of adherent leukocytes and platelets in cerebral venules. This response was not altered by splenectomy. LPS also caused a significant decrease in circulating leukocytes, which was unchanged by splenectomy. There was no affect on circulating platelets. These results demonstrate that splenectomy does not protect the brain from non‐sterile cerebral inflammation and the protective effects of splenectomy on the inflamed brain appear to be specific to stroke. Support or Funding Information This work was supported by a fellowship from the Center for Cardiovascular Diseases and Sciences, LSU Health Sciences Center‐Shreveport