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Bone Marrow‐Derived Stromal Cells (BMSCs) Improve Microvascular Perfusion in Skin Flaps Subjected to Ischemia Reperfusion Injury (IRI)
Author(s) -
Tang Ya Hui
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.721.2
Subject(s) - medicine , perfusion , blood flow , ischemia , stromal cell , reperfusion injury , surgery , wound healing , transplantation , skin flap , bone marrow , artery , blood supply , pathology
Skin flaps are frequently used for the functional and cosmetic repair of wounds and defects left behind after trauma, burns, and tumors. Flap survival mainly depends on the blood supply provided through re‐established vessels of the dermal and subdermal plexus. We have previously demonstrated the efficacy of BMSCs in the enhancement of wound healing and in improving flap survival. Objective To determine if the intravascular delivery of BMSCs improve blood perfusion in the skin flaps following IRI. Methods A cutaneous flap model perfused via the inferior epigastric artery was created in wild type (C57Bl/6), which underwent 3.5 hours of ischemia, followed by reperfusion and BMSCs transplantation. Blood perfusion in the flap was monitored using a noncontact, real‐time and noninvasive Laser Speckle Contrast Analysis (LSCA) system. Flap survival and BMSCs recruitment were also assessed. Results The numbers of trans‐migrated BMSCs appearing in the flap interstitial spaces were increased, compared to control (sham) mice, within 4h after IRI. Blood flow, both proximal and distal to the vascular pedicle was marked increased (p<0.01) in postischemic flaps treated with BMSCs treatment, when compared to IRI controls at 3 days of reperfusion. Conclusion The results of this study indicate that the enhanced wound healing and improved flap survival observed following BMSC treatment may be related to an improvement in tissue blood flow. Support or Funding Information Supported by a Malcolm Feist Postdoctoral Fellowship