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Adipocyte derived factors affect cardioprotective programs initiated by short term high fat feeding
Author(s) -
Haar Lauren,
Luther Kristin,
Ren Xiaoping,
Wang Yang,
Jones WK
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.717.5
Subject(s) - cardioprotection , viability assay , adiponectin , adipocyte , adipose tissue , autophagy , chemistry , oil red o , medicine , microbiology and biotechnology , endocrinology , biology , pharmacology , adipogenesis , biochemistry , insulin , cell , ischemia , insulin resistance , apoptosis
Rationale High fat mediated cardioprotection is a form of protection that utilizes short‐term high fat diet to decrease infarct size post ischemia/reperfusion injury. This protection is dependent on the increase of autophagy related proteins and the activation of NF‐κB mediated gene transcription. This study addresses the hypothesis that signaling from adipose tissue can remotely influence the cardioprotective markers associated with high fat mediated (HFD) cardioprotection. Methods Hormonal activation of cardiac receptors was assessed through treatment with murine recombinant adiponectin and AdipoRon peptide. Isolation of adipocyte derived exosomes was also preformed from 3T3‐L1 (ATCC® CL‐173) cells during pre differentiation, mitotic expansion, and post differentiation states. Viability of cells pretreated with each of the previously described agents was then assessed with Hoescht 33342 and propridium iodide staining before and after the administration of an oxygen glucose assay (OGD) protocol. Autophagy measurements were performed with LC‐3A/B and Beclin‐1 western blot, and the Premo Autohpagy Sensor® transduction for each treatment condition. In vivo studies were preformed in wild type C57/B6J and AdipoQ‐KO murine models, with in vivo LAD occlusion (30m) and reperfusion (24h). Conclusion Exosomes isolated from 3 phases during the differentiation of 3T3‐L1 (ATCC® CL‐173) cells into mature adipocytes did not increase the viability of cells after OGD treatment. Post OGD protocol, AdipoRon (25uM) treatment improved viability and cell survival relative to vehicle treated H9c2(2‐1) (ATCC® CRL‐1446) controls. Total serum levels of adiponectin were increased by an average of 15% in 24h HFD wild type animals relative to control fed wild type animals, and AdipoQ‐KO animals lacked cardioprotection at previously reported cardioprotective high fat fed time points. Future studies will focus on the assessment of in vivo hormonal signaling during high fat mediated cardioprotection.