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Kinetics and Implications of Germinal Center Formation in Induced Bronchus‐Associated Lymphoid Tissue During Influenza Infection
Author(s) -
GibsonCorley Katherine N.,
Boyden Alexander W.,
Tygrett Lorraine,
Waldschmidt Thomas J.
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.700.5
Subject(s) - germinal center , immune system , immunology , flow cytometry , biology , lymphatic system , antigen , acquired immune system , b cell , antibody
Induced bronchus‐associated lymphoid tissue (iBALT) develops within the lung of both humans and mice following an adaptive immune response upon exposure to certain classes of antigens. iBALT has been documented to develop with Influenza A virus (IAV) infection but the kinetics of this localized immune response have yet to be documented. Using both flow cytometry and immunohistochemistry we have identified the structure, composition and development of B cells and iBALT over time in mice intranasally infected with an H1N1 IAV. Early in infection (days 6–12) B cells accumulate within the lung, primarily around blood vessels, and are very loosely organized. By flow cytometry, these are mature B cells and there is no evidence of germinal center cells. As time progresses to days 18 and 24, B cells more tightly organize around medium to large airways and have a well‐defined lymphoid structures complete with Bcl‐6 positive, PNA hi , CD95 hi , IgM hi germinal center B cells which persist up to and likely beyond day 50 of infection. These findings are of interest as Influenza viruses are constantly undergoing antigenic changes, which make the development of vaccines very challenging. Understanding the kinetics of iBALT development can help us identify key triggers for a protective adaptive immune response and novel targets for vaccine development. Support or Funding Information Departmental Funding; Department of Pathology, University of Iowa