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Renal subcapsular space of Balb/c nude mice as a route to evaluate differentiation of human bladder carcinoma T24 cells subpopulations
Author(s) -
Souza Diego Mota,
Matheus Luiz Henrique Gomes,
Oliveira Brito Rodrigo Barbosa,
Matos Yves Silva Teles,
Malta Camila Soares,
Ferreira Janaina Mendes,
Silva Chrisna Souza,
Dellê Humberto
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.697.4
Subject(s) - pathology , bladder cancer , metastasis , vimentin , cancer cell , cytokeratin , nude mouse , renal capsule , mesenchymal stem cell , immunohistochemistry , medicine , cancer , transplantation
Recurrence of bladder cancer after resections with progression to more aggressive forms are critical to promote cancer‐specific death and metastasis. Subpopulations of cancer cells, including cancer stem‐like cells, have been found inside the bladder tumors, having different abilities for malignancy and chemotherapy resistance. Subpopulations have been described and isolated from a culture of T24 cells, which represent major human epithelial bladder cancer cell with clinical relevance. However, in situ influences can not be assessed by another way except by animal model. Since T24 cells are not tumorigenic in nude mice when inoculated subcutaneously and technical limits exist in orthotopic procedures, we evaluated the renal subcapsular space as an alternative route to analyze these cells in vivo . Balb/c nude mice underwent renal subcapsular inoculation of 1×10 6 T24 cells, suspended in two different volumes of PBS (50 μl and 10 μl). Four weeks post‐inoculation, grafted kidneys were harvested and fixed for histology and immunohistochemistry analysis. Other tissues were investigated for metastasis. Inoculation of the cells in a volume of 50 μl was unsuccessful. However, a very high success rate was reached when 10μl volume was used (87.5%). It was possible to identify the formation of a pseudo‐bladder structure on renal subcapsular space, as well as a clear cellular differentiation, which was immunohistochemically characterized for epithelial to mesenchymal transition (cytokeratin for epithelial cells and vimentin for mesenchymal cells). Subpopulations of T24 cells were found infiltrating the renal parenchyma toward the medulla, besides surrounding renal vessels. Renal subcapsular inoculation is an effective route to analyze subpopulations and differentiation of T24 cells in vivo . Support or Funding Information FAPESP (2012/04423‐0)