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Preclinical safety profile of a depleting antibody against CRTh2 for asthma: Well tolerated despite unexpected CRTh2 expression on vascular pericytes in the central nervous system and gastric mucosa
Author(s) -
Austin Cary D.,
Huang Tao,
Sharma Neeraj,
Liu Shan,
Solon Margaret,
Reyes Arthur,
Paul Sarah,
Yee Angie,
Tao Janet,
Chalasani Sreedevi,
BienLy Nga,
Barck Kai,
Carrano Richard A.D.,
Wang Jianyong,
Rangell Linda,
Bremer Miere,
Danilenko Dimitry M.,
Katavolos Paula,
Hotzel Isidro,
Reif Karen,
Rajapaksa Kathila S.
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.694.2
Subject(s) - pericyte , antibody , immune system , medicine , blood–brain barrier , immunology , immunohistochemistry , pathology , central nervous system , biology , endothelial stem cell , biochemistry , in vitro
CRTh2 is expressed on immune cells that drive asthma pathophysiology. Current treatment options for severe asthma are inadequate and therapeutic antibody‐mediated depletion of CRTh2‐expressing cells represents a promising new therapeutic strategy. Here we report for the first time that CRTh2 is not only expressed on immune cells, but also on microvasculature in the central nervous system (CNS) and gastric mucosa in humans. Microvascular expression of CRTh2 raises a safety concern because a therapeutic anti‐CRTh2 antibody with enhanced depletion capacity could lead to vascular damage. To evaluate this safety risk, we characterized microvascular expression in human and in transgenic mice expressing human CRTh2 protein (hCRTh2.BAC.Tg) and found that CRTh2 is not localized to microvascular endothelium that is directly exposed to circulating therapeutic antibody, but rather, to pericytes that in the CNS are shielded from direct circulatory exposure by the blood‐brain barrier. Immunohistochemical visualization of an intravenously administered anti‐CRTh2 antibody in transgenic mice revealed localization to microvascular pericytes in the gastric mucosa but not in the CNS, suggesting the blood‐brain barrier effectively limits pericyte exposure to circulating therapeutic antibody in the CNS. Repeated dosing with a depleting anti‐CRTh2 antibody in hCRTh2.BAC.Tg mice revealed linear pharmacokinetics and no drug‐related adverse findings in any tissues, including the CNS and gastric mucosa, despite complete depletion of CRTh2 expressing circulating eosinophils and basophils. Collectively, these studies demonstrate that the likelihood of drug‐related CNS or gastrointestinal toxicity in humans treated with a therapeutic depleting anti‐CRTh2 antibody is low despite pericyte expression of CRTh2 in these tissues.