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Zinc Transporter 7 ( Znt7 ) Knockout in Mice Differentially Affects Lipid Metabolism in Adipose Tissues
Author(s) -
Huang Liping,
Tepaamorndech Surapun,
Kirschke Catherine P.,
Pedersen Theresa L.,
Keyes William R.,
Newman John W.
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.691.4
Subject(s) - lipogenesis , endocrinology , medicine , adipose tissue , lipid metabolism , adipocyte , knockout mouse , fatty acid , lipid droplet , carbohydrate metabolism , biology , chemistry , biochemistry , receptor
Znt7 ( Slc30a7 , zinc transporter 7) knockout (KO) mice are mildly zinc deficient, accompanied with low body weight gain and body fat accumulation. Immunohistochemistry study revealed that ZnT7 protein was more abundantly expressed in the mouse subcutaneous fat than the epididymal (visceral) fat. To investigate whether Znt7 KO had differential effects on fat metabolism in subcutaneous and epididymal fat depots, we examined fatty acid composition and insulin sensitivity in both fat depots isolated from Znt7 KO and control mice. We showed that Znt7 KO had differentially adverse effects on fatty acid metabolism and insulin action in the subcutaneous fat depot but not in the epididymal fat depot, consistent with the ZnT7 protein expression pattern in the tissue. Furthermore, we found that ZnT7 protein expression was significantly up‐regulated by lipogenic differentiation and ZnT7 expression reached a peak when 3T3‐L1 cells were fully differentiated. We further demonstrated that 3T3‐L1 adipocytes with Znt7 expression 70% knocked down had low activations of Akt and Erk1/2 upon lipogenic stimulation leading to low glucose uptake and lipid synthesis in adipocytes. Our findings strongly suggest a role for ZnT7 in adipocyte lipogenesis. Support or Funding Information This work was supported by the United States Department of Agriculture, ARS CRIS projects 2032‐51000‐004‐00D and 2032‐51530‐022‐00D.