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In Vitro Effects of Grape Phenolics, Myricetin and Piceatannol, on Bovine Granulosa Cell Proliferation and Steroid Production
Author(s) -
Spicer Leon J,
Schutz Luis F,
Zhang Lingna
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.691.35
Subject(s) - chemistry , myricetin , piceatannol , growth factor , in vitro , steroid , endocrinology , medicine , hormone , biology , biochemistry , resveratrol , antioxidant , quercetin , kaempferol , receptor
Myricetin (MYRI) and piceatannol (PICE) are naturally occurring phenolic compounds in red wine with cardio‐protective, anti‐carcinogenic, and anti‐osteoporosis effects. However, potential reproductive effects of MYRI (a flavonol) or PICE (a stilbenoid) have not been investigated in any animal species. The objective of the present study was to determine if MYRI and PICE can directly affect ovarian function using bovine granulosa cells (GC) as an in vitro model system to evaluate effects on GC proliferation and steroid production. Ovaries from non‐pregnant cattle were collected from a local slaughterhouse and follicular fluid was aspirated from small follicles (1–5 mm) to isolate GC. GC were cultured for 2 days in 10% fetal bovine serum followed by 2 days in serum‐free medium containing 500 ng/ml of testosterone (as an estradiol precursor), 30 ng/ml of follicle stimulating hormone and insulin‐like growth factor 1 (IGF1; 0 or 30 ng/ml) with MYRI (0, 1 or 30 μM) or PICE (0, 1 or 30 μM). These experiments were replicated with three independent pools of GC and treatments applied in triplicate for each pool. Data were analysed as a 2 (with/without IGF1) × 3 (3 doses of MYRI or PICE) factorial ANOVA. Statistical analyses revealed that both IGF1 and dose of MYRI and PICE significantly affected estradiol and progesterone production. IGF1 increased (P < 0.05) GC numbers (2‐fold) and estradiol (4‐fold) and progesterone (1.3‐fold) production. MYRI and PICE at 30 μM (but not at 1 μM) completely blocked IGF1‐induced progesterone production. In contrast, MYRI significantly stimulated IGF1‐induced estradiol production by 1.8‐fold, whereas PICE at 30 μM (but not at 1 μM) inhibited IGF1‐induced estradiol production by 90% (P < 0.05). Neither MYRI nor PICE significantly affected GC numbers. In conclusion, the red wine phenols, MYRI and PICE, affected GC steroidogenesis, and suggests that they may have the ability to impact reproductive function. Support or Funding Information Supported in part by the Oklahoma State University Agricultural Experiment Station.