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Proteomic analysis of human serum of osteopenic women after dried plum treatment
Author(s) -
Lee Fanny,
Ghassemian Majid,
Schenk Simon,
Kern Mark,
Hooshmand Shirin
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.691.32
Subject(s) - bone remodeling , proteome , acid phosphatase , alkaline phosphatase , medicine , endocrinology , proteomics , bone mineral , chemistry , biochemistry , osteoporosis , enzyme , gene
Dried plum has the ability to prevent and reverse bone loss as evidenced by an increase in bone mineral density as well as changes in bone turnover biomarkers such as insulin growth factor‐1, bone‐specific alkaline phosphatase, tartrate‐resistant acid phosphatase‐5b and sclerostin. However, it is not known whether dried plum affects other proteins in human serum that may influence bone turnover. The objective of this study was to explore if dried plum affects proteins in human serum other than the known bone turnover biomarkers related in the reversal of bone loss in osteopenic postmenopausal women using isobaric tags for relative and absolute quantification (iTRAQ). Serum samples were obtained from forty‐eight osteopenic women (65–79 years old) who were randomly assigned to one of three treatment groups for six months: 1) control 2) 100g dried plum or 3) 50g dried plum. Samples were collected at baseline and after three and six months of intervention. The iTRAQ quantitative proteomics approach was conducted to explore the proteome affected by dried plum treatment. iTRAQ results identified 51 unique proteins that were upregulated with at least a 2‐fold difference in change between control and treatment groups (p<0.05). Twenty‐four proteins were significantly upregulated only at 3‐months, five proteins at both 3‐ and 6‐months and eighteen at 6‐months only. Of the relevant proteins, ten proteins relate to inflammation, at least 5 relate to immunological function in general, five relate to lipid metabolism and one has a known direct association with bone metabolism. Dried plum treatment resulted in an upregulation of 51 unique proteins in human serum, which may or may not be linked to bone turnover. Future studies must be conducted to determine if these are in fact related to bone turnover processes. Support or Funding Information Pilot Award from UCSD CTRI (UL1 TR000100)