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Lycopene Induces Apoptosis in Pancreatic Cancer Cells
Author(s) -
Jeong Yoonseon,
Lim Joo Weon,
Kim Hyeyoung
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.691.23
Subject(s) - survivin , apoptosis , inhibitor of apoptosis , pancreatic cancer , cancer research , cancer cell , viability assay , lycopene , mtt assay , xiap , cancer , programmed cell death , chemistry , caspase , biology , medicine , biochemistry , carotenoid
Pancreatic cancer is one of the most fatal human cancers, which has the lowest 5‐year survival rate among all cancer types. Lycopene, a major carotenoid component in tomatoes, has a potential anticancer activity in many types of cancers. The inhibitors of apoptosis (IAPs) including survivin, cellular inhibitor of apoptosis 1(cIAP‐1) and cellular inhibitor of apoptosis 2(cIAP‐2) are a group of anti‐apoptotic factors in the apoptotic pathway that cause cancer cells become insensitive to apoptotic stimulation. The purpose of this study is to investigate the anti‐cancer mechanism of lycopene by determining the expression levels of inhibitors of apoptosis in human pancreatic cancer cells, PANC‐1. The cells were treated with various concentrations of lycopene or caspase‐3 inhibitor Z‐VAD_FMK. Cell viability was examined by MTT assay. Expressions of survivin, cIAP‐1 and cIAP‐2 were determined by real‐time PCR. We found that the cell viability was decreased by lycopene. A caspase inhibitor Z‐VAD_FMK suppressed lycopene‐induced cell death. In addition, lycopene decreased mRNA expression of survivin, cIAP1 and cIAP2 in pancreatic cancer cells. These results indicate that lycopene induces the apoptosis of pancreatic cancer cells by suppressing the expression of survivin, cIAP1 and cIAP2. Lycopene may be a promising therapeutic agent for human pancreatic cancer.