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Effects of Resveratrol Derivatives on Plasma Membrane Ca 2+ ‐ATPase and Ca 2+ Homeostasis in MDA‐MB‐231 Human Breast Cancer Cells
Author(s) -
Peterson Joshua Allen,
Kenealey Jason Donald
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.691.22
Subject(s) - plasma membrane ca2+ atpase , cytosol , resveratrol , calcium , calcium metabolism , chemistry , homeostasis , atpase , extracellular , biochemistry , calcium pump , apoptosis , microbiology and biotechnology , biology , enzyme , organic chemistry
Resveratrol (RES), a naturally occurring polyphenol found in grapes, peanuts, and berries, selectively facilitates apoptosis in cancer cells. The apoptotic activity of RES in tumor cells is, in part, due to a large, sustained increase in cytosolic calcium ion concentration. In properly functioning cells, plasma membrane Ca 2+ ‐ATPase (PMCA) extrudes excess calcium from the cytosol to the extracellular space. PMCA prevents toxically high levels of calcium from accumulating in the cytosol and maintains cytosolic calcium homeostasis. Via a recently developed PMCA activity assay that utilizes live cell fluorescence imaging, we found that RES inhibits PMCA. Because we have seen in past experiments that altering the hydroxyl groups in RES modifies the cytosolic calcium response, we performed a structure‐function experiment to determine which RES hydroxyl groups are most important to PMCA inhibition. In order to identify the structurally important components of RES, we conducted PMCA activity experiments using RES derivatives p‐hydroxystilbene, pinosylvin, piceatannol, and oxyresveratrol. We have determined the role of RES hydroxyl groups in inhibiting PMCA activity in the human breast cancer cell line MDA‐MB‐231. Support or Funding Information Brigham Young University, College of Life Sciences Startup Grant