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Metabolomic analysis revealed dose‐dependent association between growth performance and redox metabolites in algae feeding
Author(s) -
Ma Yiwei,
Zhou Wenguang,
Chen Paul,
Urriola Pedro E,
Shurson Gerald C,
Ruan Rogen,
Chen Chi
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.690.23
Subject(s) - algae , metabolome , biology , metabolism , metabolomics , glutathione , lipid metabolism , biochemistry , methionine , carnitine , polyunsaturated fatty acid , food science , fatty acid , botany , amino acid , metabolite , bioinformatics , enzyme
Algae are an enriched source of proteins, polyunsaturated fatty acids, vitamins, and dietary fibers, but the impacts of algae feeding on the metabolome were not well characterized. In this study, metabolic effects of were evaluated by feeding (n=24) with three different treatments. The metabolic status of young male mice fed with 0%, 5%, and 20% (w/w) fresh water algae ( Scenedesumus spp.) was evaluated by growth performance, blood chemistry, and LC/MS‐based metabolomics. The growth performance was significantly increased by 5% algae while decreased by 20% algae feeding. Serum glucose, triglyceride, and BUN levels were not affected by both treatments but cholesterol dramatically decreased by 20% algae feeding. Metabolomic analysis of liver, serum, feces and urine samples indicated that algae feeding greatly affected the metabolites belonging to amino acid, lipid, microbial metabolism and antioxidant system. The growth promotion effect of 5% algae feeding was associated with the increased levels of hepatic reduced glutathione, niacinamide, dephophocoenzyme A, and adenylsuccinic acid. In contrast, the growth suppression effects of 20% algae feeding was correlated to the increased level of oxidized glutathione and carnitine in the liver, increased EPA and DHA in liver and serum, and increased acyl‐glycine in the urine. Overall, the results suggested that low level of algae feeding may up‐regulate antioxidant system and anabolic metabolism while high‐level exposure could disrupt the redox balance and lipid metabolism.