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Postprandial Cranberry Flavonol Glycoside Bioavailability in Human Urine
Author(s) -
Nelson Heather N,
Kaiser Amanda J,
Reker Nicolette C,
Wang Yifie,
Singh Ajay P,
Vorsa Nicholi,
Hooks Tisha L,
Wilson Ted
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.690.19
Subject(s) - postprandial , chemistry , urine , bioavailability , proanthocyanidin , glycoside , quercetin , ingestion , food science , pharmacology , biochemistry , polyphenol , medicine , endocrinology , diabetes mellitus , antioxidant , organic chemistry
Cranberry health benefits for prevention of urinary tract infections have been linked to proanthocyanidins even though their bioavailability is questionable. Protection could also be dependent upon the bioavailability of cranberry flavonol glycosides, which have not been previously characterized in urine postprandially. Healthy women (n=10; 19.5 ± 1.1 years; 22.5 ± 2.0 BMI) ingested low‐acidity 27% v/v cranberry juice (CBJ) from Demoranville cranberries sweetened with sucrose (74 Cal/240 ml), or an isocaloric control (74 Calories sucrose/240 ml). Midstream urine was collected at baseline (0), then 90, 225, and 360 minutes postprandially. UHPLC‐ESI‐MS‐MS methods were developed to identify flavonol glycosides in CBJ and urine. CBJ quercetin‐3‐galactoside (Q‐3‐gal), quercetin‐3‐arabinoside (Q‐3‐ara), myricetin‐3‐galactoside (M‐3‐gal), quercetin‐3‐rhamnoside (Q‐3‐rha), and myricetin‐3‐arabinoside (M‐3‐ara) concentrations were 1.8, 0.5, 0.5, 0.3, and 0.2 mg/240ml. Postprandial peak concentration times in urine were 114.5 ± 12.1, 237.2 ± 12.1, 90.0 ± 12.1, 151.4 ± 12.1 and 104.4 ± 12.8 min, respectively. These flavonol glycosides were not detected in control (0 min) urine. Following CBJ ingestion, Q‐3‐gal/mg creatinine was the most abundant flavonol glycoside at 1298 ± 421, 302 ± 85, 74 ± 59 (ng/mg creatinine) at 90, 225 and 360 min. Q‐3‐rha was 597 ± 206, 444 ± 142, and 122 ± 66. Q‐3‐ara was 36 ± 20, 337 ± 92, and 226 ± 96. M‐3‐ara was 270 ± 102, 56 ± 23, and 19 ± 15. M‐3‐gal was 169 ± 28, 8 ± 28 and 5 ± 28. This is the first study demonstrating that CBJ flavonol glycosides occur in urine postprandially. Sugar conjugate specificity at the 3‐linkage appears to affect flavonol glycoside bioavailability. Future studies should consider whether Q‐3‐gal or other flavonol glycosides may be responsible for UTI protection.