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The physical stability comparison of two types of resveratrol nanocarriers
Author(s) -
Zu Yujiao,
Wang Shu
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.690.10
Subject(s) - zeta potential , dispersity , particle size , nano , nanocarriers , chromatography , chemistry , aqueous solution , analytical chemistry (journal) , nanoparticle , nuclear chemistry , materials science , nanotechnology , organic chemistry , composite material
Objective Resveratrol (R) has anti‐obesity bioactivities, but its low level of aqueous solubility and stability limits its application. We successfully synthesized biocompatible and biodegradable R encapsulated lipid nanoparticles (R‐nano) and R encapsulated liposomes (R‐lipo). The objectives of this study are to measure the characteristics of R‐nano and R‐lipo and to compare their physical and chemical stability at three different temperatures. Method The particle size and polydispersity index (PI) of R‐nano and R‐lipo were measured using a Brookhaven BI‐MAS particle size analyzer, and their zeta potential were measured using a Brookhaven ZetaPALS analyzer. The R‐nano and R‐lipo were stored in dark at 4°C, 22°C and 37°C for 7 days, and their physical and chemical stability was measured every 24 hours. The freshly made R‐nano and R‐lipo were aliquot into black tubes and stored at the above three temperatures. The mean particle size, PI and zeta potential were measured every 2 hours for the first 10 hours, and every 24 hours for 7 days. The chemical stability of R‐nano, R‐lipo and native R were measured by a high‐performance liquid chromatography system. Results The mean particle size of freshly made R‐nano and R‐lipo were around 140 nm and 110 nm, respectively. The PI values of both R‐nano and R‐lipo were less than 0.3. Moreover, the zeta potential of R‐nano and R‐lipo were around −19 and −28mV. As compared to native R, R‐nano and R‐lipo dramatically increased the aqueous solubility of R by more than 30 times. Nanoencapsulation also significantly enhanced the chemical stability of R. The diameter and zeta potential of R‐lipo did not changed significantly at all temperatures (4°C, 22°C and 37°C) for 7 days, and the PI of R‐lipo remained under 0.3. R‐nano had lower physical stability than R‐lipo at 22°C and 37°C. After incubating at 22°C for 24 hours and at 37°C for 8 hours, the particle size was dramatically increased, and their PI and zeta potential values were also increased. The particle size, PI and zeta potential of R‐nano were stable at 4°C for 24 hours. Conclusions Both R‐lipo and R‐nano increased the aqueous solubility and stability of R. R‐lipo had higher physical stability than R‐nano. We are investigating the anti‐obesity bioactivities of nanoencapsulated R. Support or Funding Information Grant Funding Source: NIH 1R15AT008733‐01