Premium
Pyrogallol, a microbial metabolites from mango tannins ( Mangifera Indica L.) suppresses breast cancer ductal carcinoma in situ proliferation in both in vitro and in vivo
Author(s) -
MertensTalcott Susanne U,
Kim Hyemee,
Barnes Ryan,
Talcott Stephen T,
Nemec Matthew
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.688.7
Subject(s) - pyrogallol , breast cancer , cancer , metabolite , mangifera , chemistry , polyphenol , in vivo , pharmacology , cancer research , biochemistry , medicine , biology , botany , microbiology and biotechnology , antioxidant
It is estimated that 1 in 10 newly diagnosed cancer cases will be female breast cancer, and therefore there is an ongoing need to research novel treatment options. Polyphenolics are secondary plant metabolites that have been shown to have anti‐cancer effects in multiple cancer models without the deleterious side effects of conventional small molecules. This research, focused on the anti‐proliferative activities of pyrogallol, an intestinal microbial metabolite of mango polyphenols. Mice were xenographed with MCF10DCIS.COM cells in order to determine the anti‐proliferative of pyrogallol on ductal carcinoma in situ breast cancer (DCIS). Treatment animals received either 0.2mg/day or 0.8mg/day pyrogallol, and both treatment groups had significantly decreased tumor volumes compared to the control animals after 4 weeks of treatment. Plasma and tumor tissue was assessed in order to determine in pyrogallol or its metabolites were present in circulation or at the site of action. Additionally, biomarkers along the AMPK‐mTOR signaling axis were affected at mRNA, protein and phosphorylated protein levels. These results suggest that anti‐proliferative activities of mango polyphenols may be mediated by pyrogallol as a central bioactive metabolite that is systemically absorbed. Findings indicate that a diet rich in gallotannins that are microbially metabolized to pyrogallol may prevent the advancement of DCIS breast cancer. Support or Funding Information Mango National Board