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Anti‐tumor Properties of Beta‐sitosterol in AGS Human Gastric Adenocarcinoma cells and Tumor Xenograft Mice
Author(s) -
Shin Eunju,
Kim Sung Hee,
Hwang JinTaek
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.688.11
Subject(s) - ampk , pten , apoptosis , cancer research , chemistry , dna fragmentation , cancer cell , cell , cancer , microbiology and biotechnology , programmed cell death , biology , medicine , pi3k/akt/mtor pathway , phosphorylation , biochemistry , protein kinase a
Although beta‐sitosterol (BS), one of several phytosterols, has been proposed to be effective for improving human health, the precise molecular mechanisms of BS regarding anti‐cancer effects have not yet been elucidated. Here, we investigated effects of BS regarding apoptotic mechanisms. BS significantly induced cell death in a dose dependent manner. These effects were accompanied by typical appearances of apoptosis including DNA fragmentation, apoptotic assay, caspase3/7 activity, mitochondria membrane potential and activation of tumor suppress genes including PTEN and AMPK in AGS cells. Inhibition of AMPK by compound C (AMPK inhibitor) rescued PTEN expression induced by BS. These results suggest that stimulation of PTEN expressions by BS is dependent on AMPK signaling. Furthermore, BS at 100mg/(kg · day) also inhibited tumor growth in tumor xenografts. Taken together, BS exerts potent anti‐cancer properties by modulating AMPK and PTEN in AGS cells as well as xenograft mice models.