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Korean Pine Nut Oil Replacement decreases intestinal lipid uptake while improves hepatic lipid metabolism in High‐Fat Diet Fed Mice
Author(s) -
Shuang Zhu,
Park Soyoung,
Lim Yeseo,
Shin Sunhye,
Han Sung Nim
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.684.6
Subject(s) - medicine , chemistry , endocrinology , cd36 , lipid metabolism , metabolism , apolipoprotein b , fatty acid , very low density lipoprotein , steatosis , lipoprotein , biology , biochemistry , cholesterol , gene
Consumption of pine nut oil (PNO) was shown to reduce weight gain and attenuate hepatic steatosis in mice fed with high fat diet (HFD). In this study, we aimed to explore the effects of PNO on both intestinal and hepatic lipid metabolism in mice fed a HFD. Five‐week‐old C57BL/6 mice were fed control diets containing 10% energy fat from either Soybean Oil (SBO) or PNO (SC or PC groups), or HFD (45% energy from fat) containing 15% energy fat from lard and 30% energy fat from SBO or PNO (SHF or PHF groups) for 12 weeks. Expression of genes related to intestinal fatty acid (FA) uptake and channeling ( Cd36 , Fatp4 , Acsl5 , Acbp ), intestinal chylomicron synthesis ( Mttp , ApoB48 , ApoA4 ), hepatic lipid uptake and channeling ( Lrp1 , Fatp5 , Acsl1 , Acbp ), hepatic triacylglycerol (TG) lipolysis and FA oxidation ( Atgl , Cpt1a , Acadl , Ehhadh , Acaa1 ), as well as very low‐density lipoprotein (VLDL) assembly ( ApoB100 ) were determined by real‐time PCR. Overall, replacement of SBO with PNO resulted in significantly less body weight gain ( P <0.05). In intestine, PNO‐fed mice had significantly lower Cd36 mRNA expression ( P <0.05). ApoA4 mRNA levels were significantly lower in PHF compared with SHF ( P <0.05). In addition, PNO consumption tended to result in higher hepatic mRNA levels of Atgl ( P =0.08) and Cpt1a ( P= 0.051). The mRNA levels of Acadl and ApoB100 were significantly higher in mice fed PNO diet (both P <0.05). Together, lower Cd36 and ApoA4 mRNA expression in PNO consumption groups suggest that PNO may decrease activities of intestinal FA uptake and chylomicron assembly in intestine. The tendency of higher Atgl and Cpt1a mRNA expression, together with the significantly higher Acadl and ApoB100 mRNA expression in PNO‐fed group may imply that PNO could increase hepatic TG metabolism; mitochondrial fatty acid oxidation and VLDL assembly. In conclusion, PNO replacement may function to prevent excessive lipid uptake by intestine as well as improve hepatic lipid metabolism in HFD fed mice. Support or Funding Information Supported by the grant from the National Research Foundation (NRF) of Korea (NRF‐2010‐0024878)