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Korean Fermented Soy Sauce Modulate Na + /K + ‐ATPase Activity in HK‐2 Cells
Author(s) -
Cha YounSoo,
Mun EunGyung,
Kim Mina,
Yook Jinseon
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.682.5
Subject(s) - fermentation , chemistry , reabsorption , sodium , osmole , homeostasis , tonicity , biochemistry , food science , epithelial sodium channel , biophysics , endocrinology , biology , organic chemistry
Na + /K + ‐ATPase (NKA) is membrane transporter in mammals. The proximal tubules of kidney play a key role in the maintenance of ionic homeostasis functioning via Na + and water reabsorption. Modulation of NKA is an important mechanism for animal cells to survive in diverse environments, especially in the compensation of hypertonicity‐induced disturbances. HK‐2 cells are human renal proximal tubule cell line; it has NAK in apical membrane which functions as the first step to absorb the Na + . Also, NAK, Na + /H + exchanger isoform 3 (NHE3), Na + /Ca 2+ exchanger (NCX), Na + /HCO 3 − cotranspoter (NBC), and carbonic anhydrase (CA) control the absorption of salt. Korean fermented soy sauce including doenjang and ganjang , fermented from meju using Bacillus and Aspergillus species of bacteria. Fermentation of soybeans alters the profiles of isoflavonoids, small peptides, and urea cycle intermediated. However, fermented soy sauce contains high‐salt, which secure the long‐term fermentation. This problem can be health problem in high‐salt intake such as hypertension. HK‐2 cells were exposed to hypertonic condition (600 mOsm/kg H 2 O), and the relative NKA activity were analyzed via spectrophotometer. NKA activity was increased in HK‐2 cell upon hypertonic challenge in time‐dependency. NHE3 activity was also increase such as NKA activity in various concentration of hypertonic condition (400~600 mOsm/kg H 2 O) for 24 hours. Korean fermented soy sauce modulates NKA and NHE3 activities in HK‐2 cells. Support or Funding Information This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education(2015R1D1A3A01015869)