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Caffeine Consumption and its Association with Mitochondrial Specific Oxidative Stress in People Living with HIV in the Miami Adult Studies on HIV Cohort
Author(s) -
Ramamoorthy Venkataraghavan,
Campa Adriana,
Sales Sabrina,
Rubens Muni,
Fleetwood Christina,
Stewart Tiffanie,
Alvarado Glenda,
Khan Hafiz,
Liuzzi Juan P.,
George Florence,
Baum Marianna
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.668.13
Subject(s) - caffeine , oxidative stress , medicine , cohort , physiology , cohort study , human immunodeficiency virus (hiv) , immunology
Objective High caffeine consumption has been associated with compromised serum antioxidant nutrients in people living with HIV (PLWH). This could adversely affect mitochondrial oxidative status. This study examines the relationship between caffeine consumption and a marker of mitochondrial oxidative stress. Methods After consenting 130 PLWH on stable antiretroviral therapy recruited from the Miami Adults Studies on HIV (MASH) cohort, caffeine intake was measured using standardized questionnaires. Blood was drawn for mitochondrial‐specific 8‐oxo‐2′‐deoxyguanosine (mt‐specific 8‐oxo‐dG), a marker of mitochondrial oxidative stress. CD4 cell counts and HIV viral load were obtained from medical records with written permission from participants. SPSS version 21 was used for statistical analyses. More than 250 mg of caffeine intake or the equivalent of 3–4 cups of coffee daily was considered the cut‐off point for high consumption. This study was approved by the Florida International University Institutional Review Board. Results Mean age of this group was 47.9 ± 6.4 years, 60.8% were men, and 75.4% were Black. Mean caffeine intake was 337.63 ± 304.97 mg/day. Levels of mt‐specific 8‐oxo‐dG were higher in participants reporting >250 mg/day of caffeine consumption (0.40±0.34 vs 0.54±0.40 ΔCt [=Ct 2 – Ct 1 ], P =0.049). High caffeine consumption was significantly correlated with high mt‐specific 8‐oxo‐dG levels (r=0.212, P =0.028), and significantly predicted higher mt‐specific 8‐oxo‐dG levels (β=0.693, P =0.049), adjusting for CD4 counts and viral load. Conclusion High caffeine intake adversely affected mitochondrial‐specific oxidative status in PLWH in the MASH cohort. Further studies are needed to elucidate the bio‐chemical mechanisms for these adverse effect. Support or Funding Information Funded by NIDA and FIU Graduate School Dissertation Year Fellowship.