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Scant interactions of biomarkers of oxidative stress and anti‐oxidation defense with those of the inflammatory response among preschool children form the Western Highlands of Guatemala
Author(s) -
Méndez Maria José Soto,
Aguilera Concepción María,
Mesa Maria Dolores,
Rico María Cruz,
CampañaMartín Laura,
MartínLaguna Victoria,
Solomons Noel W.,
Schuemann Klaus,
Gil Angel
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.668.11
Subject(s) - superoxide dismutase , glutathione peroxidase , medicine , biomarker , oxidative stress , glutathione reductase , antioxidant , urine , endocrinology , cotinine , chemistry , physiology , immunology , biochemistry , nicotine
Objective To explore the presence and magnitude of paired associations between diagnostic biomarkers of oxidation and inflammation from the same or different anatomical compartments. Methods 82 children, 38 girls and 44 boys (median age 56 mo), attending 3 daycare centers from a government‐subsidized system in Quetzaltenango, Guatemala, delivered single samples of urine, whole blood, saliva, and feces for one or more of the selected determinations. We were able to make pairing correlations of Among 23 biomarker variables, paired Spearman rank‐order correlations could be made from 12 from the inflammatory domain (White blood cells, plasmatic and salivary IL‐1β, IL‐6, IL‐8, IL‐10 and TNFα, and fecal calprotectin) and 11 from the oxidation domain (urinary markers of oxidation {15‐Isoprostane‐F2 t and 8‐hydroxy‐deoxy‐guanosine}; erythrocyte activity of antioxidant enzymes {catalase, superoxide dismutase, glutathione reductase and glutathione peroxidase}; and plasmatic concentrations of antioxidant nutrients {retinol, tocopherols, β‐carotene and Coenzymes Co‐Q 9 and Co‐Q 10 }). The SPSS version 20 software program was used. Results Within the 132‐cell full matrix, 13 statistically‐significant associations were found (9.8% of possible), seven at a p‐value of <0.01 and 6 from <0.05 to 0.01. Superoxide dismutase and β‐carotene were each associated with 5 and 6 inflammatory biomarkers, respectively. Backward‐elimination multiple‐regression analyses with both as dependent variables showed the same two predictors, plasmatic TNF‐α and saliary IL‐8, with an r 2 ‐value of 0.155 for superoxide dismutase and r 2 0.136 for β‐carotene. Conclusion Significant associations in an inter‐class context between paired, oxidation‐vs‐inflammation biomarker variables represent only a 10 th of the possible associations and a fraction of the 33–57% association seen with the same data, previously analyzed on an intra‐class hemi‐matrix basis. Support or Funding Information Funded by: Fundación Iberoamericana de Nutrición (FINUT), Spain and The Hildegard Grunow Foundation (HGF), Germany