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PGC‐1α‐mediated Changes in Phospholipid Profiles of Exercise‐trained Skeletal Muscle
Author(s) -
Miura Shinji,
Senoo Nanami,
Miyoshi Noriyuki,
GotoInoue Naoko,
Minami Kimiko,
Yoshimura Ryoji,
Morita Akihito,
Sawada Naoki,
Matsuda Junichiro,
Ogawa Yoshihiro,
Setou Mitsutoshi,
Kamei Yasutomi
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.659.1
Subject(s) - skeletal muscle , phospholipid , coactivator , phosphatidylethanolamine , endocrinology , mitochondrial biogenesis , medicine , glycolysis , biology , oxidative phosphorylation , mitochondrion , chemistry , biochemistry , phosphatidylcholine , metabolism , transcription factor , gene , membrane
Exercise training influences phospholipid fatty acid composition in skeletal muscle and these changes are associated with physiological phenotypes; however, the molecular mechanism of this influence on compositional changes is poorly understood. Peroxisome proliferator‐activated receptor γ coactivator 1α (PGC‐1α), a nuclear receptor coactivator, promotes mitochondrial biogenesis, the fiber‐type switch to oxidative fibers, and angiogenesis in skeletal muscle. Because exercise training induces these adaptations, together with increased PGC‐1α, PGC‐1α may contribute to the exercise‐mediated change in phospholipid fatty acid composition. To determine the role of PGC‐1α, we performed lipidomics analyses of skeletal muscle from genetically modified mice that overexpress PGC‐1α in skeletal muscle or that carry knockout alleles of PGC‐1α. The overexpression of PGC‐1α in the skeletal muscle caused a significant change in the phosphatidylcholine (PC) and phosphatidylethanolamine (PE) profiles of the muscle. The PC and PE profile of EDL from PGC‐1α‐Tg mice, which showed oxidative characteristics, was similar to the profile of the originally oxidative muscle, such as the soleus. We also found that PGC‐1α increased several phospholipid species in glycolytic muscle, namely PC (18:0/22:6) and PE (18:0/22:6). Exercise training increased PC (18:0/22:6) and PE (18:0/22:6) in glycolytic muscle and that PGC‐1α was required for these alterations. Since phospholipid fatty acid composition influences cell permeability and receptor stability at the cell membrane, these phospholipids may contribute to exercise training‐mediated functional changes in the skeletal muscle. Support or Funding Information This study was supported by the Council for Science, Technology and Innovation (CSTI), Cross‐ministerial Strategic Innovation Promotion Program (SIP, No.14533567), and “Technologies for creating next‐generation agriculture, forestry and fisheries” (funding agency: Bio‐oriented Technology Research Advancement Institution, NARO), The Tojuro Iijima Foundation for Food Science and Technology (Chiba, Japan), Grants‐in‐Aid for Scientific Research (KAKENHI, No. 26282184, 26560400, 21300240) from the Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT, Tokyo), The Uehara Memorial Foundation (Tokyo, Japan), The Kao Research Council for the Study of Healthcare Science (Tokyo, Japan, No. A‐31006), and University of Shizuoka Grant for Scientific and Educational Research.