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TRANSCRIPTOME ANALYSIS OF ILLINOIS ABDOMINAL AND CARDIAC ADIPOSE
Author(s) -
Schneider Blair Kathleen,
Kern Colin,
Hubbard Allen,
Treible Wayne,
Finger John W.,
Tuberville Tracey,
Glenn Travis C.,
Hamilton Matt,
Lamont Susan J.,
Schmidt Carl J.
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.654.2
Subject(s) - transcriptome , biology , adipose tissue , gene , computational biology , rna seq , genetics , gene expression , bioinformatics , endocrinology
The domestic chicken is very important in its use as a model organism for understanding regulation of numerous biological processes in avians. To better understand energy storage and mobilization, this work focuses on defining the transcriptome of chicken abdominal and cardiac adipose tissue. Transcriptome libraries were prepared from Illinois abdominal and cardiac adipose samples with the following procedure: 1. Isolation of mRNA using mir Vana™ miRNA Kit, 2. Verification of mRNA quality and integrity by Fragment Analysis, 3. Transcriptome libraries prepared using Illumina Stranded RNA Prep Kit and sequenced at the Delaware Biotechnology Institute Core Sequencing Facility, 4. Transcription levels determined using the fRNAkenseq software, and 5. Identification of differentially expressed genes, data analysis, functional clustering and pathway analysis. To begin our analysis we identified genes that were differentially expressed between the abdominal and cardiac fat tissue. This identified differentially expressed transcripts encoding adipokines, transcription factors and enzymes involved in processing fat. The same procedure was performed on alligators, and the gene expression was analyzed in their fat bodies. Current effort focuses on comparing alligator gene expression with that obtained from chicken adipose tissue to better understand the evolution of this tissue in Archosaurs. Support or Funding Information Acknowledgements: This material is based upon work supported by the National Science Foundation under Grant No. 1147029 and by the Agriculture and Food Research Initiative Competitive Grant No. by NIFA 2010‐04233 from the USDA National Institute of Food and Agriculture.This Figure shows a hierarchical clustering of the alligator and Illinois (chicken) cardiac and abdominal adipose samples. The grouping was based on the gene expression of all the genes present. With one exception, the alligator samples clustered together while the chicken abdominal and cardiac samples formed separate clusters.