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Microarray analysis and anti‐hyperglycemia effect of Gryllus bimaculatus (GB, a type of cricket) extract in rats on high fat diet
Author(s) -
Ahn Mi Young,
Hwang Jae Sam,
Yun Eun Young
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.654.1
Subject(s) - endocrinology , medicine , chemistry , gryllus bimaculatus , triglyceride , malondialdehyde , fatty acid , cholesterol , food science , biochemistry , biology , oxidative stress , ecology , cricket
Wistar rats fed a high‐fat diet (HFD) were orally administered with phosphate buffered saline (PBS, control), GB (100 mg/kg or 200 mg/kg), Provastatin or Isaria sinclairii (IS) extract, which is reported to have fat‐reducing effects, for either 1 or 2 months. GB's sero‐biochemial, hematological and anti‐oxidizing hepato‐cellular biomarker levels were evaluated to determine their anti‐lipidemic, anti‐inflammatory, and anti‐coagulant effects after 1 month of GB treatments in HFD (fat 60%) Wistar rats. The abdominal and epididymidal fat weights were measured and the composition of fatty acid was analyzed by GC/MS. The high fat dieted Wistar rats treated with GB showed increases in the unsaturated fatty acids (FA) ratio, especially with single (mono) FA. However, there were decreases in saturated fatty acids. Microarray analyses were performed with a rat 28K cDNA clone set array to identify the gene‐expression profiles for the GB exposed high fat dieted Wistar rat. The weight and fatty acid composition of abdominal fat and epididymidal fat, total cholesterol, LDL‐cholesterol, and triglyceride in GB treated rats were at lower levels than those of the control group. The anti‐oxidant hepato‐cellular biomarker levels, protein carbonyl content and malondialdehyde concentrationin GB treated rats were significantly decreased. Compared to the control, the GB treated rat group (treated at a dose of 100 and 200 mg/kg), had 190 up‐regulated genes including Gpm6a, Tmem14a and Fasin, with 235 down‐regulated genes including Cc121b, Glycan1 and Tcrb. The data suggest that Fasin‐related fatty acid synthesis and adipose differentiation related protein (Adfp), which is related to obesity, were upregulated by GB treatment, indicating their potential as therapeutic markers for anti‐atherosclerosis or inflammation. Support or Funding Information ASBMB member