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Effects of Gryllus bimaculatus (Gb) fractions on alcoholic hepatitis
Author(s) -
Ahn Mi Young,
Hwang Jae Sam,
Yun Eun Young
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.653.11
Subject(s) - ethanol , alcohol , chemistry , chloroform , alkaline phosphatase , alcoholic hepatitis , fraction (chemistry) , transaminase , medicine , glycosaminoglycan , butanol , endocrinology , chromatography , biochemistry , alcoholic liver disease , enzyme , cirrhosis
In the present study, several Gb fraction samples derived from ethanol extracts of Gb crickets, such as Gb butanol fraction, Gb ethylacetate fraction, Gb chloroform fraction, and Gb hexane fraction (100 mg/kg each), were examined for their liver protective effects against alcoholic hepatitis in SD rats. We used silymarin 40 mg/kg as a positive control. Animals in each group were intraperitoneally given glycosaminoglycan (5 mg/kg or 10 mg/kg), followed by treatment of each fraction sample. One hour later, 1.5 mL of 30% ethanol was orally administered to all groups. The above procedure was repeated for consecutive 3 days. Then, animals were selected to collect their blood samples for blood chemistry analysis, and the following results were obtained using plasma samples harvested: The serum alcohol concentration decreased by 0.08%, when treated with PBS only. However, the serum levels of alcohol in groups treated with Gb etylacetate fraction, Gb chloroform fraction, and hexane fraction decreased by 0.06%, 0.02% and 0.01%, respectively. The loss of alcohol concentrations was observed in the groups treated with 5 mg/kg and 10mg/kg of glycosaminoglycan derived from Gb, and the levels of alanine transaminase (ALT) also decreased. Serum ethanol concentrations decreased in the Gb ethylacetate 100 mg/kg group, and anti‐alcoholic hepatitis effects through inhibition of hyaluronate secretion were also noted. In the chloroform fraction group, anti‐alcohol accumulation effects in the body were found through the loss of plasma concentrations of ethanol, hyaluronate, alkaline phosphatase, and ALT. Serum alcohol levels were reduced in the Gb hexane fraction group. When each of Gb glycosaminoglycan at a dose of 5 or 10 mg/kg was administered to rats for consecutive 3 days, four serum parameters (ethanol, alkaline phoshosphatase, AST, and ALT) showed significant losses in alcohol absorption. The results suggest that Glycosaminoglycan may improve alcoholic hepatitis. We could also confirm the decreased levels of creatinine, hyaluronate and free fatty acid in the blood. Support or Funding Information ASBMB member