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Dibutyltin Exposures Alter Secretion of Interleukin 6 from Human Immune Cells
Author(s) -
Brown Shyretha,
Wilburn Wendy,
Whalen Margaret
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.651.5
Subject(s) - secretion , peripheral blood mononuclear cell , immune system , tumor necrosis factor alpha , monocyte , interleukin , cytokine , chemistry , immunology , biology , endocrinology , medicine , pharmacology , biochemistry , in vitro
Dibutyltin (DBT) is an organotin compound that is used as a stabilizer in polyvinyl chloride (PVC) plastics (including pipes that distribute drinking water and bottles) and as a de‐worming agent in poultry. DBT is found in human blood samples, and DBT exposures alter the secretion of tumor necrosis factor alpha (TNFα) from lymphocytes. IL‐6 is an anti‐inflammatory agent, as well as a pro‐inflammatory mediator, that is produced by T lymphocytes and monocytes. It is responsible for immune response regulation as well as tissue repair and cellular growth. The aim of the current study is to determine whether exposure to DBT alters the secretion of IL‐6 from increasingly reconstituted preparations of human immune cells. We examined whether exposure to DBT concentrations of 0.05 to 5 μM after 24h, 48h, or 6 days alters IL‐6 secretion in a preparation of highly enriched human NK cells, a monocyte‐depleted preparation of human peripheral blood mononuclear cells (PBMCs) (MD‐PBMCs), PBMCs, granulocytes, and a preparation combining both PBMCs and granulocytes. The levels of IL‐6 were monitored using an enzyme‐linked immunosorbent assay (ELISA). The results indicate that DBT alters IL‐6 secretion from all cell preparations. The concentrations ranging from 0.5 μM to 5 μM caused diminished IL‐6 secretion, while the lowest concentration caused elevation of IL‐6 secretion. The concentrations and lengths of exposure to DBT that caused statistically significant increases in IL‐6 secretion from human immune cells varied from one donor to the next. Therefore, the data indicates that DBT‐induced alterations of IL‐6 secretion from immune cells may be a significant consequence of DBT exposures that may potentially affect immune competence. Support or Funding Information Supported by NIH grant 5U54CA163066