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Effect of Pathway‐Interconnectors in SEB‐Induced Apoptosis Related Events in Human PBMCs
Author(s) -
Geddes Erin,
Hendricks Michelle,
Doyle Connor,
Borgos Dylan,
Burton Danielle,
Mendis Chanaka
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.647.2
Subject(s) - peripheral blood mononuclear cell , apoptosis , gene , signal transduction , kinase , enterotoxin , microbiology and biotechnology , immunology , chemistry , biology , cancer research , in vitro , genetics , escherichia coli
Staphylococcus enterotoxin B (SEB) is produced by the bacterium Staphylococcus aureus. SEB is a common cause of apoptosis within human peripheral blood mononuclear cells (PBMCs). The purpose of this study is to further investigate human PBMCs in order to better understand the apoptosis related events induced by SEB. C‐Jun N‐Terminal Kinase (JNK) has been previously identified to be induced by SEB, and has demonstrated that it inter‐connects multiple signaling cascades. As a crucial pathway inter‐connector in SEB‐induced human PBMCs, we believe that inhibiting JNK may possess inhibitory effects on SEB‐induced apoptosis. In this study, JNK specific inhibitor (SP600125) was used to inhibit JNK. We will further evaluate the gene expression profile using RT and PCR. Protein expression patterns of some genes will be examined through the use of enzyme‐linked immunosorbent assays (ELISAs). We believe that our work will allow us to better understand the complex interactions of multiple signal transduction pathways induced by SEB. Support or Funding Information We want to thank James Waldschmidt, Tricia Mason, Aaron Hopkins, Emily Freehauf, Amanda Smet, and Nicole Krausert who worked on different aspects of the project. We also wanted to thank our Collaborator Dr. Marti Jett, MEDCOM, Frederick, MD. The project was funded by Centerpoint and PURF, SAIF grants (University of Wisconsin ‐Platteville).