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Curcumin Inhibits Development and Cell Adhesion in Dictyostelium discoideum : Implications for YakA Signaling and GST Enzyme Function
Author(s) -
Garige Mamatha,
Walters Eric
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.644.12
Subject(s) - dictyostelium discoideum , curcumin , microbiology and biotechnology , biology , signal transduction , cell adhesion , dictyostelium , extracellular matrix , cell growth , biochemistry , cell , gene
The molecular basis for nutraceutical properties of the polyphenol curcumin (Curcuma longa, Turmeric) is complex, affecting multiple factors that regulate cell signaling and homeostasis. Here, we report the effect of curcumin on cellular and developmental mechanisms in the eukaryotic model, Dictyostelium discoideum . Dictyostelium proliferation was inhibited in the presence of curcumin, which also suppressed the prestarvation marker, discoidin I, members of the yakA‐mediated developmental signaling pathway, and expression of the extracellular matrix/cell adhesion proteins (DdCAD and csA). This resulted in delayed chemotaxis, adhesion, and development of the organism. In contrast to the inhibitory effects on developmental genes, curcumin induced gstA gene expression, overall GST activity, and generated production of reactive oxygen species. These studies expand our knowledge of developmental and biochemical signaling influenced by curcumin, and lends greater consideration of GST enzyme function in eukaryotic cell signaling, development, and differentiation. Support or Funding Information Department of Biochemistry and Molecular Biology, Howard University College of Medicine, Washington D.C.