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Parental diet modulates gene expression in Drosphila melanogaster
Author(s) -
Tresser Jason
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.628.3
Subject(s) - offspring , longevity , biology , drosophila melanogaster , epigenetics , gene , gene expression , genetics , genomic imprinting , dna methylation , pregnancy
Caloric restriction is known to promote longevity in various animal species presumably through modulations in various metabolic pathways. In fruit files, low yeast content in food (low protein diet) has been shown to significantly extend lifespan. Microarray studies in Drosophila indicate that in addition to changes in the expression of metabolic genes, epigenetic factors also play a role in longevity. This suggests the possibility that offspring from diet restricted flies may inherit a propensity for long life through genetic imprinting. In our study, we are using quantitative PCR to measure gene expression levels in the offspring of flies subjected to diet restricted. In particular, the NAD+ ‐dependent deacetylase Sir2 is known to be upregulated in diet restricted flies. We are measuring Sir2 levels in offspring at embryonic, larval and adult stages.