Premium
Characterization of Basigin and MCT1 expression in murine myocardium
Author(s) -
Morse Grace E,
Ochrietor Judith D
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.627.11
Subject(s) - basigin , immunoglobulin superfamily , antibody , biology , inflammation , glycoprotein , gene , microbiology and biotechnology , immunology , genetics , matrix metalloproteinase
Obesity is a condition that has systemic effects that include hypertension, diabetes, and atherosclerosis. The long‐term goal of this study is to examine the effects of obesity and inflammation in the mouse heart, specifically looking for potential biomarkers that can be used to predict cardiovascular disease long before its onset. Previous reports have established Basigin, a glycoprotein member of the immunoglobulin superfamily (IgSF), as a heterotypic cell‐adhesion molecule expressed in two splice‐variant forms varying in the expression of extracellular immunoglobulin (Ig) domains. Basigin variant‐2 and Basigin variant‐1 contain two and three C2 domain loops, respectively. The variant‐2 protein is known to be expressed in mouse heart. Monocarboxylate transporter 1 (MCT1), a proton‐linked lactate transporter, known to interact with Basigin gene products is expressed in murine myocardium during vascular remodeling following atherosclerotic conditions. Therefore, the purpose of this present study was to investigate the relative expression of Basigin and MCT1 gene‐products in murine myocardium during post‐natal development. Mouse hearts were obtained via an accepted protocol and protein lysates were generated. Enzyme‐linked immunosorbent assay (ELISA) was utilized to determine the relative expression of Basigin and MCT1 in murine myocardium samples at several ages. It was determined that expression of both molecules increases over the life of the animal. We concluded that expression of Basigin and MCT1 in murine myocardial tissue may be potential biomarkers for cardiovascular disease. Support or Funding Information University of North Florida Office of Undergraduate Studies and the Office of Academic Affairs