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Whole‐Blood Optimization of Electrochemical Biosensors for Biomedical Diagnostics
Author(s) -
Daniel Jessica,
McCoy Michael,
Bonham Andrew J
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.604.3
Subject(s) - biosensor , aptamer , nanotechnology , polyethylene glycol , point of care testing , chemistry , materials science , computer science , biochemistry , microbiology and biotechnology , medicine , biology , immunology
Electrochemical aptamer‐scaffold biosensors are able to detect target proteins, such as botulism and ricin toxins, in small concentrations with reliable results. Although these rationally designed biosensors perform well in buffered media under lab‐bench conditions, this is insufficient for real world diagnostic performance. Thus, we have developed methods utilizing polyacrylamide hydrogels and hydroxyl‐polyethylene glycol‐thiol passivants to optimize these biosensors for function with both small sample volumes and in whole blood, delivering rapid and accurate analysis. Through these surface optimizations and the creation of rapid software analysis front‐ends, we have created a platform for usage of this class of biosensors that successfully functions in complex media and delivers rapid, clear results for toxins in their diagnostic range. This work will enable greater adoption of electrochemical biosensors in industrial fields and as point‐of‐care diagnostics.