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A Preliminary Study on Serum Proteomics in Yin‐Deficiency‐Heat Syndrome Rats using iTRAQ‐coupled 2DLC‐MS/MS
Author(s) -
Liu Changming,
Mao Liangen,
Ping Zepeng,
Jiang Tingting,
Wang Chong,
Chen Zhongliang,
Xu Dandan,
Li JiCheng
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.603.3
Subject(s) - decoction , chinese herbs , medicine , apolipoprotein b , traditional chinese medicine , endocrinology , chemistry , normal group , pathology , cholesterol , alternative medicine
This study aimed to observe the expression profiles of serum proteins and to discover potential biomarkers in Yin‐deficiency‐heat (YDH) syndrome rats induced by hot and pungent Chinese herbs. Totally 50 Sprague–Dawley rats were randomly divided into the normal group and the YDH model group. The concentrated decoction of three Chinese herbs such as aconite root, dried ginger, and cinnamon were applied to establish YDH model. The signs and symptoms of rats were observed dynamically. All rats were sacrificed at the end of the 4th week and serum was collected by using carotid artery retaining needle. Serum protein profiles in YDH rats and normal rats were investigated by iTRAQ‐coupled 2DLC‐MS/MS technique. Compared with the normal group, rats in the YDH model group showed obvious YDH symptoms, including dry hair, restlessness, and weight loss. Rats in the YDH model group gained weight more slowly (p<0.01) than did those of the normal group, and the thymic index decreased significantly (p<0.001) in the YDH model group. When setting quantification ratio of >1.25 as up‐regulated threshold and <0.8 as down‐regulated threshold, a total of 165 differentially expressed proteins (110 up‐regulated proteins and 55 down‐regulated proteins) were identified in the serum of YDH syndrome rats. Significant differences in protein Kininogen (KNG), Apolipoprotein C‐III (ApoC‐III) and Paraoxonase1 (PON1) were found between the YDH model group and the normal group by ELISA. Our work provided valuable insights into the changes of the protein expression in YDH syndrome rats, and identified 3 differentially expressed proteins (KNG, ApoC‐III and PON1) that can be used as potential biomarkers for diagnosing YDH syndrome. Support or Funding Information This work was supported by grants from National Basic Research Program of China (No.2014CB543002), National Special Sci‐Tech Projects (No. 2012ZX10005001‐006), and National Natural Science Foundation of China (No.81273882, NO. 81573709).