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A Novel Immunoassay, AGILE Research, Enables Detection of Analyte in Quantity Limited Samples
Author(s) -
Barron Francie Elizabeth,
Scannura Ryan,
Tavares Andre LP,
Walker Amy,
Pan Deng,
Lerner Mitch,
Clouthier David E,
Goldsmith Brett
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.602.4
Subject(s) - analyte , proteome , proteomics , pooling , computational biology , computer science , rna , quantitative proteomics , sample (material) , protein expression , nanotechnology , biology , chemistry , bioinformatics , materials science , gene , chromatography , biochemistry , artificial intelligence
Proteomics work can be sample‐limited, making some experiments difficult to impossible to complete. One example is proteome analysis in mouse embryos, in which limited protein yield from embryonic tissue results in the requirement of sample pooling to obtain sufficient material for analysis. Due to this sample limitation and expense, RNA studies are often utilized to assess changes in gene expression, with the assumption that protein levels should concomitantly change with RNA levels. To overcome these obstacles, we have developed a novel direct detection approach that leverages advances in nanoelectronic sensors to quantify protein concentrations. AGILE Research is a new protein binding analysis tool using graphene biological field effect transistors that eliminates the need for secondary labeled antibodies to obtain results, and it can detect proteins in the fM range in as little as 15 minutes via a simple USB connection. We show data from use of AGILE Research demonstrating protein concentration from in vivo and in vitro quantity limited samples.