Premium
Genomic Analysis of the Resistance of E. coli to Benzyl Bromide
Author(s) -
Qi Macee,
Muenter Mark,
Baig Samir,
Bellou Sirine,
Carlson Alyssa,
Conway Charles,
Joshi Christopher,
Kramer Caitlin,
Leifer Becky,
Travers Meghan,
Wang Poguang,
Giese Roger,
Beuning Penny
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.576.1
Subject(s) - dna damage , dna , alkylation , dna repair , chemistry , gene , complementation , carcinogen , biochemistry , biology , mutant , catalysis
Exposure to an alkylating agent can occur via industrial exposure or treatment in cancer chemotherapy as cytotoxic agents, causing DNA alkylation. DNA alkylation is a common occurrence in which alkyl groups are covalently attached to DNA bases resulting in DNA lesions. DNA lesions can arise either from endogenous sources through metabolism or exogenously from environmental exposure. The resulting lesions can cause mutations that can lead to cancer. Therefore understanding the DNA alkylation process and identifying the major DNA adducts that form from specific alkylating agents is important. The overall aim of our research is to determine the survival of E. coli AB1157 cells when treated with benzyl bromide (BnBr), an alkylating agent used in the chemical and pharmaceutical industries. The survival of wild‐type E. coli AB1157 and twenty‐seven derivatives, each harboring a knockout of a specific gene associated with various DNA repair processes has been determined upon exposure to the alkylating agent BnBr. In order to confirm whether the deletion of specific genes in E. coli that showed resistance when exposed to the alkylating agent is indeed the cause of decreased survival, complementation studies are being carried out. Benzylation profiles for free nucleosides (dA, dT, dC, dG) reacted with BnBr have been determined using HPLC‐MS. Analyses of various DNA samples exposed to BnBr are being carried out to characterize the major adducts formed, allowing us to determine how DNA repair pathways contribute to repairing the specific adducts.