Novel Gene Identification Using the Transpose Elements in Rhodococcus aetherivorans Plasmid pRA3

Genome annotation has a huge impact on molecular biology. Therefore, making an annotated database using Rhodococcus aethreivorans bacteria will allow researchers to have databanks that they can base upon in their new research. Rhodococcus aethreivorans bacteria has not been annotated before and there is almost no coding in bacterial molecular biology. This database identifies certain elements called transpose elements that encode important proteins and also play a critical role in evolution. In order to create this database, inserting the pRA3 script was the first step. The second step is marking the open frames which label genes in this plasmid. The third step is using BLAST(Basic Local alignment tool)search tool, which is the main tool that is used to identify genes. The fourth step is navigating the start of the sequence to know where the protein actually stats in order to make the data base more accurate. Also, this annotation of this single plasmid took a month and a half to be completed and 9 new elements were discovered. Other genomes may take up to 6–12 months. Thus, this will save scientist a lot of time. In order to further develop this study, each new element will be studied alone to understand it. Also, the annotation of other bacteria such as streptococcus pneumonia, Helicobacter pylori, Staphylococus aureus and Streptococcus mutans will be completed.Circular visualization of the whole plasmid pRA3Graph shows high GC contentGraph shows low GC content