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Neuritin, a New Potential Target for Optic Nerve Injury Therapy
Author(s) -
Xu Jiajun,
Cao Wenluo,
Liu Weiye,
Ma Haoqian,
Qi Aiqing,
Huang Tingting,
Li Lin,
Liu Fang
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.564.3
Subject(s) - optic nerve , retina , neurotrophic factors , downregulation and upregulation , neurite , neuroscience , neurotrophin , axotomy , retinal ganglion cell , central nervous system , microbiology and biotechnology , biology , medicine , in vitro , receptor , biochemistry , gene
Optic nerve, which also belongs to the central nervous system, is difficult to repair once get injured. Neuritin, a novel member of the neurotrophic factor family, serves as a common downstream effector of neurotrophic factors and neural activity. Our experiment investigated the spatiotemporal expression of neuritin in adult rat retina and optic nerve in normal and after optic nerve crush. Following crush, neuritin protein and mRNA transiently increased in retina and optic nerve. In vivo, after neuritin lentivirus infection of rat retina, the number of retinal ganglion cells and optic nerve axons were increased significantly after injury, this profective effects was further confirmed by the functional tests like pupillary light reflex and visual evoked potential, suggesting that neuritin play a critical role in optic nerve regeneration. Further more, our results showed exogenous rhneuritin promotes RGC5 cell line survival and neuritin overexpression promotes neurite outgrowth which may through the upregulation of phosphorylation of Akt and Erk, and reduces cell apoptosis which may through the inhibition of caspase 3 activation after RGC5 damage in vitro. Our experiments suggested that neuritin is a potential new target for optic nerve injury therapy and provide alternative way for repairing injury of central nervous system. Support or Funding Information National Natural Science Foundation of China (Grant No. 31271282)