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Thymoquinone Enhances Neurogenesis to A Greater Extent in Middle‐Aged Than in Young Aged Rat in Chronic Epilepsy
Author(s) -
Brito Sijam Alassaf,
Rao Muddanna Sakattu
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.561.8
Subject(s) - neurogenesis , thymoquinone , kainic acid , neun , medicine , hippocampal formation , lesion , hippocampus , dentate gyrus , epilepsy , neuroprotection , doublecortin , neurodegeneration , endocrinology , anesthesia , pathology , neuroscience , psychology , chemistry , glutamate receptor , immunohistochemistry , disease , biochemistry , psychiatry , antioxidant , receptor
Neurogenesis in chronic epilepsy is known to be decreased significantly in a young aged, middle aged and aged rats. Thymoquinone (TQ), an active constituent of Nigella sativa seeds possesses anti‐inflammatory, neuroprotective properties and reported to enhance the neurogenesis in different CNS disease models. Aim of the study was to compare the efficacy of TQ in neuro protection immediately after status epileptics (SE), suppression of spontaneous recurrent motor seizures (SRMS) and aberrant mossy sprouting, and enhancement of neurogenesis, in young (4 months) and middle aged (12 months) chronically epileptic rats. SE was induced by intraventricular injection of kainic acid (0.5μg/ventricle) bilaterally in 4 months old and 12 months old male wistar rats(n=12 for each age group). These lesioned rats were treated with TQ (10 mg/kg; ip) for 4 days. A subgroup of lesion only (LO) and lesion+ TQ (L+TQ) rats were sacrificed at 5 th day for analysis of hippocampal neurodegeneration by staining the brain sections with flurojade‐B. Remaining rats were video monitored between 2.5–3.0 months post SE for quantifying spontaneous recurrent motor seizure. Lesion only, Lesion+ TQ rats were sacrificed at the end of 3 rd month along with normal and sham control rats. (n=6/group). Brain sections were stained with Fluro‐Jade B and labeled for doublecortin. The number newly born neurons and mossy fiber sprouting were quantified in the dentate gyrus at the end of 3 rd month. TQ significantly decreased SRMS, reduced the neuron degeneration (25%), in CA1, CA3 and dentate hilus, suppressed the mossy fiber sprouting (30–40%) both in 4 months and 12 months age groups. TQ treatment increased the neurogenesis in both groups, however in middle aged rats it is significantly more than in young aged rats (P<0.001). Our results suggest that TQ is more effective in enhancing the neurogenesis in middle aged chronic epileptic rats than young aged chronic epileptic rats. Support or Funding Information ( Supported by Kuwait University Grant #YM02/14 ).