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LIPIDIC AGENT AS VASCULAR CONSTRAST‐MEDIUM FOR MRI (3T) IN FETAL AND EMBRYONIC DEVELOPMENT: FEASABILITY‐STUDY ON EX‐VIVO RABBIT FETUS AT 3T
Author(s) -
TONNELET Romain,
PEREZ Manuela,
GONDIM TEIXEIRA Pedro Augusto,
SEWONU Anou,
BEAUMONT Marine,
HOSSU Gabriela,
LABROUSSE Marc,
BRAUN Marc
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.554.1
Subject(s) - ex vivo , fetus , biomedical engineering , in vivo , medicine , pathology , chemistry , biology , pregnancy , microbiology and biotechnology , genetics
Understanding some dynamic processes such as the development of the cardiovascular system requires a global and precise anatomic vision of this dynamic and complex process A variety of imaging tools and reconstructions are available for the evaluation of embryonic development. Micro‐MRI was used as the “standard” technique in large study series 1, 2 competing with micro‐CT and techniques requiring the preparation of the sample in serial sections (EFIC method 3 / 3D reconstruction from serial histological sections : EmbryoForm Project 4 ). Material & Method Embryonic imaging is also restricted by the use of vascular MRI contrast agents such as gadolinium coupled to albumin, which are particularly expensive. Lipidic agents have high viscosity at ambient temperatures and a T1 shortening effect for vascular rendering. Due to the non‐diffusion phenomenon related to its inherent hydrophobic properties, lipidic agents are restricted to the intra‐vascular compartment. These characteristics make lipidic agents a potentially available and cost effective option for embryonic vascular imaging. In this study we sought to evaluate the use of a lipidic contrast agent (Type‐B oil / Fisher Scientific) using dedicated coils with a 3Tclinical MR scanner. A qualitative analysis of the injected vascular anatomical structures and a quantitative analysis by the contrast‐to‐noise ratio (CNR) and signal‐to‐noise ratio (SNR) was performed on two rabbit fetuses. Results Large and medium vessels structures including thoraco‐obdominal great vessels, intra‐hepatic vasculature and vascular brain structures were identified by two trained operators. CNR and SNR measures were in agreement with those of the literature. Such a technique have the potential to increase the availability of embryonic vascular imaging and facilitate research in the field of vasculogenesis.