Premium
Epigenetic signatures of alcohol abuse in hepatocellular carcinoma
Author(s) -
Puszyk William Matthew,
Hlady Ryan,
Robertson Keith,
Cabrera Roniel,
Liu Chen
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.516.11
Subject(s) - cyp2e1 , hepatocellular carcinoma , alcohol , cell culture , alcohol dehydrogenase , epigenomics , in vitro , cancer research , liver cell , alcohol abuse , medicine , biology , chemistry , gene expression , gene , biochemistry , dna methylation , genetics , psychiatry , microsome
There is a paucity of human in vitro models available to study the effects of alcohol in hepatocellular carcinoma (HCC). HCC cell lines are often deficient in either alcohol dehydrogenase (ADH) or cytochrome P450 2E1 (CYP2E1). VL‐17a is currently the most widely studied model available. VL‐17a is derived from a HepG2 cell line engineered to overexpress mouse ADH and human CYP2E1. LH86 is a novel HCC cell line previously developed in our laboratory. Here we compare the LH86 cell line with the current model to identify a novel tool to study the effects of alcohol in HCC. The aim of this study is to evaluate the LH86 cell line as a novel human in vitro model to study the effects of alcohol in HCC. The cell lines were cultured with alcohol and gene expression and epigenomic changes were identified. The LH86 cell line was observed to have unique functional properties indicating that it is a potential novel tool to study the effects of alcohol in HCC. Support or Funding Information R01 AA19976 TO KR AND CL