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Black Berry Polyphenol Reduce Nox1 Function to Inhibit Senescence in Vascular Smooth Muscle Cells
Author(s) -
Huang Jingwen,
Feresin Rafaela,
Zhao Yitong,
Pourafshar Shirin,
Arjmandi Bahram H.,
Salazar Gloria
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.420.2
Subject(s) - nox1 , senescence , reactive oxygen species , nadph oxidase , vascular smooth muscle , superoxide dismutase , antioxidant , oxidative stress , chemistry , polyphenol , superoxide , angiotensin ii , western blot , biochemistry , ageing , microbiology and biotechnology , biology , medicine , endocrinology , enzyme , receptor , smooth muscle , gene
The consumption of fruits and vegetables has been shown to decrease cardiovascular diseases (CVD) incidence mainly due to the polyphenol content in these foods. Angiotensin II (Ang II) plays an important role in inducing ageing‐related diseases including CVD as it increases the production of reactive oxygen species (ROS) and reduces cellular antioxidant capacity leading to vascular senescence. The NADPH oxidase Nox1 generates superoxide and is involved in Ang II‐induced pathological effects. Objectives The purpose of this study was to investigate the role of blackberry (BL) polyphenol extracts in Ang II‐induced senescence in vascular smooth muscle cells (VSMCs) and the possible molecular mechanism involved. Methodology VSMCs treated with purified BL polyphenol extracts (200 μg/ml) were incubated with or without Ang II (100 nM). After three days of treatment, cells were analyzed for 1) senescence, measured by senescence associated β‐galactosidase (SA‐β‐gal) activity; 2) western blot to determine the activation of Ang II signaling and the expression of the senescence markers, p21 and p53, as well as antioxidant enzymes; and 3) ROS levels assessed by 2′,7′‐dichlorodihydrofluorescein diacetate (H 2 DCFDA) fluorescence. Results We found that BL polyphenol extracts prevented Ang II‐induced signaling, increased superoxide dismutase (SOD) 1 expression and reduced ROS levels preventing senescence in VSMCs. These effects were associated with down‐regulation of Nox1 expression. Further, BL prevented senescence induced by Nox1 over‐expression. Conclusions The protective role of BL polyphenols in preventing senescence is mediated by down‐regulation of Nox1. Thus, the consumption of BL could be effective in reducing the burden of CVD.