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The Impact of Limonin on Gut Microbiota
Author(s) -
Gu Min,
Sun Jin,
Qi Ce,
Cai Kun Xiao,
Goulette Tim,
Song Yue Ming,
You Meng xiao,
Sela David,
Xiao Hang
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.416.5
Subject(s) - limonin , gut flora , cecum , terminal restriction fragment length polymorphism , feces , biology , lactobacillus , microbiology and biotechnology , food science , immunology , biochemistry , restriction fragment length polymorphism , ecology , polymerase chain reaction , gene , fermentation
The gut microbiota play critical roles in human health, and diet can modulate the compositions of the gut microbiota, which in turn may contribute to altered health outcomes such as changes in the risks of chronic diseases. Limonin is one of the major bioactive compounds found in citrus fruits and have been reported to possess various beneficial health effects. This study was aimed to determine the effects of dietary limonin on the gut microbiota. Mice were fed limonin in the diet (0.05% w/w) for 5 weeks, and the microbiota were isolated for composition analysis. Terminal restriction fragment length polymorphism (T‐RFLP) patterns and Misequencing analysis were used to profile gut microbiota in the cecum, colon and feces of the mice. The results showed that compared to the control mice, treatment of limonin did not cause significant change on the diversity of the cecal microbiota, while significant changes were observed in the diversity of colonic microbiota after limonin treatment. In the colon, abundance of colonic Enterobacteriaceae was decreased by 10‐fold, while the abundance of Bifidobacteria and Lactobacillus was increased significantly in the mice treated with limonin (p<0.001). Moreover, the abundance of colonic Clostridiales was reduced to one third of that of the control mice by the administration of limonin. Overall, our study provide the first line of evidence that dietary limonin could alter gut microbiota, which warrants further investigation to determine its implication in human health. Support or Funding Information This study was partially supported by fund form USDA.