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Broccoli ( Brassica oleracea ) Extract Combats Streptozotocin‐Induced Diabetes and Oxidative Stress in Rats
Author(s) -
Suresh Sithara,
Waly Mostafa I,
Guizani Nejib,
Rahman Mohammad Shafiur
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.404.6
Subject(s) - streptozotocin , antioxidant , oxidative stress , diabetes mellitus , glutathione , glutathione reductase , glutathione peroxidase , medicine , lipid peroxidation , endocrinology , chemistry , superoxide dismutase , biochemistry , enzyme
Background The primary prevention of type 2 diabetes relies also on lifestyle changes, including dietary habits. The antidiabetic effects of several foods and dietary supplements in both animal models and in humans have been explored. It was found that beneficial effects are mainly dependent on antioxidant and hypoglycemic properties. Broccoli ( Brassica oleracea ) exerts beneficial effects in several diseases including type 2 diabetes, yet the mechanism was not elucidated. Objective This study aimed to evaluate the hypoglycemic and antioxidant potential properties of broccoli extract in diabetic rats. Methods Twenty male Sprague Dawley rats, weighting 250–300 grams, were allocated as into 4 groups (5 rats/group): non‐diabetic control that received chow diet; non‐diabetic control group that received chow diet plus oral feeding of broccoli extract; diabetic group that received chow diet; and diabetic group that received chow diet plus oral feeding of broccoli extract. Streptozotocin (STZ) drug was used as a diabetogenic agent in a single intraperitoneal injection dose of 60 gm/kg body weight, and the blood glucose level for each rat was measured twice a week. After 8 weeks, all animals were overnight fasted and sacrificed; serum was collected for measurement of glucose, and pancreatic tissues were homogenized for biochemical assessment of antioxidant enzymes (glutathione peroxidase and glutathione reductase), and oxidative stress markers (glutathione, total antioxidant capacity, lipid peroxidation, and hydrogen peroxide). Results Diabetic rats treated with broccoli extract had shown a significant protective effect against STZ‐induced hyperglycemia and oxidative stress as compared with the control group. Conclusion Our results provide evidence that broccoli extract possessed potential health benefits in controlling STZ‐induced diabetes in rats. The used in‐vivo model provides insight for the capacity of broccoli extract to be used for phytoremediation purposes in the context of human type 2 diabetes.