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High glucose culture medium downregulates production of human β‐defensin‐2 (HBD‐2) in human amniotic epithelial cells (HAEC)
Author(s) -
Szukiewicz Dariusz,
Alkhalayla Habib,
Pyzlak Michal,
Szewczyk Grzegorz
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.307.1
Subject(s) - andrology , biology , fetus , transplacental , immunology , medicine , pregnancy , placenta , genetics
Protection of materno‐placento‐fetal unit significantly depends on production of β‐defensins, including HBD‐2, by the HAEC. Regulation of innate and adaptive immunity by HBD‐2 is related to its action against gram‐negative bacteria and chemoattraction of immature dendritic cells. Despite the fact that diabetes induces placental genes for chronic stress and inflammatory pathways, the fetus is at higher risk of transplacental transmission of infection. Here we examined influence of a high glucose environment on production of HBD‐2 in cultured HAEC. Immediately postpartum, HAEC were isolated using trypsin‐based method at 37 degrees C from the amnions obtained after 48 normal full‐term pregnancies. The material was equally divided among the two groups: group I (N = 48) – cultured in high glucose culture medium (280 mg/dl), and the control group II (N = 48) – cultured in the standard medium containing 100 mg/dl glucose, in normoxia (20% O2). After pretreatment with LPS (1 mcg/ml), HBD‐2 concentrations in the HAEC culture supernatant were assessed at determined time points (120, 132, 144, and 156 hours) using ELISA. Nonparametric Wilcoxon rank‐sum test was used and the differences between the groups were considered statistically significant if p < 0.05. We observed that high glucose environment in group I was correlated with 1.1 to 2.7‐fold decrease over time in HBD‐2 concentration in the culture supernatant, compared to group II (p < 0.05). The mean viability of cells in both groups did not differ significantly at the consecutive time points. Thus, immunosupression due to hyperglycemia in poorly controlled diabetes may predispose to perinatal infections. Further studies are needed to examine whether this inhibition of HBD‐2 production is correlated with high glucose‐induced changes in the local expression of toll‐like receptors (TLRs). Support or Funding InformationSupported by WUM grant: 2M2‐W1‐15