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Fos‐Immunoreactive Neurons In Rat Intrinsic Cardiac Ganglia After Pericardial Capsaicin Injection
Author(s) -
Wang Ting,
Miller Kenneth E.
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.227.4
Subject(s) - trpv1 , capsaicin , neuroscience , sensory system , efferent , chemistry , immunostaining , transient receptor potential channel , autonomic ganglion , medicine , biology , receptor , immunohistochemistry , afferent
Intrinsic cardiac ganglia (ICG) are composed of autonomic efferent neurons, local circuit neurons, interneurons, and sensory neurons. Some sensory neurons in the ICG express transient receptor potential vanilloid type1 (TRPV1). In the current study, capsaicin, a potent TRPV1 channel agonist, was applied pericardially to stimulate the afferent nerve terminals of sensory neurons of the rat ICG. Activated, post‐synaptic ICG neurons were evaluated by Fos‐immunoreactivity. Activated, glutamatergic ICG neurons were evaluated by immunostaining for Fos and for vesicular glutamate transporter 1 (VGLUT1) or glutaminase (GLS). The results of current study demonstrated that: 1) Pericardial capsaicin is able to induce Fos‐immunoreactivity in neurons of the ICG; 2) GLS and VGLUT1‐immunoreactivity is present within some of the activated ICG neurons. 3) Fos‐immunoreactive ICG neurons, with or without GLS and VGLUT1‐ immunoreactivity, occurred in two cell size populations, small (<300μm2) and large (>300μm2). These results provide further evidence that ICG are composed of local neuronal circuitry that participate in cardiac regulation.