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Interplay between Substrate Stiffness and TGF‐b1 on Cellular Traction Force and Migration of MDA‐MB‐231 Cells
Author(s) -
LIN POYEN,
Chu YehShiu,
Lai YiTing,
Lo ChunMin
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1300.5
Subject(s) - biophysics , cell migration , chemistry , tractive force , traction (geology) , cell , materials science , microbiology and biotechnology , biochemistry , biology , physics , paleontology , thermodynamics
TGF‐β1 has been known to activate downstream signaling pathways of breast cancer cells and facilitate their invasive behaviors. In addition, our preliminary results show that MDA‐MB‐231 cells cultured on rigid substrates display higher traction forces and cell migration speed in comparison to cells cultured on more compliant substrates. The concept that a rise of contractile force due to increased ECM rigidity or TGF‐β1 induced scattering migration of MDA‐MB‐231 cells is intriguing, but the cross talk between ECM rigidity and TGF‐β1 remains to be elucidated. The goal of this study is to investigate the effect of substrate rigidity on TGF‐β1 induced changes of cell migration and traction forces of MDA‐MB‐231 cells. Collagen‐coated polyacrylamide substrates with a variety of Young's moduli (3.1, 7.4, and 10 kPa) were combined with fluorescence confocal imaging and digital image analysis to characterize a variety of cellular responses to substrate rigidity. Images of cell movement and the displacement of fluorescent beads embedded in polyacrylamide gels were taken every five minutes. Compared with control group without TGF‐β1 treatment, MDA‐MB‐231 cells on 7.4 kPa and 10 kPa gels demonstrated higher migration speed and smaller traction forces after TGF‐β1 treatment. However the addition of TGF‐β1 has little effect on traction forces or migration speed of MDA‐MB‐231 cells cultured on 3.1 kPa gels. Our results indicate that TGF‐β1 induced changes of scattering migration and traction forces are substrate rigidity dependent. Support or Funding Information This work was supported by the Ministry of Science and Technology, Taiwan. (Grant number: NSC 102‐2628‐B‐010‐010‐MY3)