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TonEBP/NFAT5 inhibits adipocyte differentiation via modulation of mitotic clonal expansion during early phase of differentiation in 3T3‐L1 cells
Author(s) -
Kim Soo Jin,
Kim Taehee,
Choi Han Na,
Kim HyunWoo,
Park Jin Bong,
Jeon Byeong Hwa,
Lee Sang Do
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1292.8
Subject(s) - adipogenesis , adipocyte , 3t3 l1 , adipose tissue , microbiology and biotechnology , endocrinology , medicine , biology , lipid droplet , cellular differentiation , transcription factor , chemistry , biochemistry , gene
Obesity resulting from excessive accumulation of white adipose tissue is closely related to the chronic diseases such as diabetes, hyperlipidemia, and hypertension. White adipose mass is determined by the number and size of adipocytes. Preadipocyte like 3T3‐L1 cells undergo terminal differentiation manifested by formation of lipid droplets as well as adipocyte specific protein. TonEBP/NFAT5 belongs to the Rel family of transcription factors and plays important roles in the development and maintenance of kidney, immunity, arthritis, and cancer metastasis. Although the functions of TonEBP/NFAT5 during osteogenesis, chondrogenesis and myogenesis are reported recently, its role in the adipogenesis is not well known. In this study, we analyzed the role of TonEBP/NFAT5 in adipogenesis using 3T3‐L1 cells. TonEBP/NFAT5 expression was dramatically reduced during adipocyte differentiation of 3T3‐L1 cells. RNAi‐mediated knock down of TonEBP/NFAT5 facilitated adipogenesis. Whereas sustained expression of TonEBP/NFAT5 using adenovirus suppressed the formation of lipid droplet and the expression of FABP4, marker for terminally differentiated adipocytes. Also, TonEBP/NFAT5 inhibited the expression of PPARγ, master regulator of terminal adipocytes. TonEBP/NFAT5 suppressed adipogenesis in the early phase of the differentiation via modulation of Akt activity and mitotic clonal expansion. These results suggest that TonEBP/NFAT5 may be an important regulatory factor in the differentiation of adipocytes. Support or Funding Information This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education (NRF‐2013R1A1A2060736, NRF‐2014R1A6A1029617)