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Age‐related arterial immune cell infiltration is attenuated by exercise or caloric restriction
Author(s) -
Trott Daniel Wayne,
Henson Grant D,
Lesniewski Lisa,
Donato Anthony
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1288.3
Subject(s) - immune system , cytotoxic t cell , cd8 , t cell , infiltration (hvac) , adipose tissue , medicine , endocrinology , immunology , chemistry , biochemistry , physics , in vitro , thermodynamics
Immune cell infiltration of the vasculature has been shown to play a role in the development of multiple cardiovascular pathologies that also occur with greater frequency in aged populations. To determine if aging increases immune cell infiltration of arteries as well as what immune cell types are involved, we tested the hypothesis that both large elastic and resistance arteries and surrounding perivascular adipose tissue in old mice would exhibit increased immune cell infiltration compared young controls. Additionally, we hypothesized that vasoprotective lifestyle interventions such as 12 weeks of voluntary wheel running or life‐long caloric restriction would attenuate age‐related immune cell infiltration. The aorta and mesenteric vasculature with surrounding perivascular adipose was excised from young (Y, 6–7 months, n = 9), old (O, 28–29 months, n = 9), old voluntary running (OVR, 28–29 months, n = 5) and old caloric restricted (28–29 months, n = 8) mice and digested to a single cell suspension. The cells were then labeled with antibodies against CD45 (total leukocytes), CD3 (pan T cells), CD4 (T helper cells), CD8 (cytotoxic T cells), CD19 (B Cells), CD11b and F4/80 (macrophages) and analyzed by flow cytometry. Total leukocytes per aorta was 5‐fold greater in O compared to Y (p < 0.001), this increase was blunted in OVR (p = 0.063 vs. Y) and completely abolished in OCR (p = 0.662 vs. Y). O aortas exhibited a 3.5‐fold increase in total T cells (p < 0.001), both helper (4‐fold, p < 0.001) and cytotoxic (3‐fold, p < 0.001) T cell subsets, B cells (7‐fold, p < 0.001) and macrophages (4‐fold, p = 0.007). The increases in T cells (p = 0.127 vs. Y), macrophages (p = 0.860 vs. Y) but not B cells (p = 0.002 vs Y) were abolished in OVR mice. Increases in T cells (p = 0.736 vs Y), macrophages (p = 0.606 vs. Y) and B cells (p = 0.692 vs. Y) were abolished OCR mice. In the mesentery, leukocytes were 2.5‐fold greater in O compared to Y both in total number (p < 0.001) and when normalized to tissue weight (3.5‐fold, p < 0.001). In a similar manner to aorta, age‐related increases in T cells and macrophages were attenuated in OVR and OCR and increases in B cells were attenuated only in OCR. These results indicate that immune cells accumulate in both the large elastic and resistance vasculature with age and that either exercise or caloric restriction can blunt this accumulation.