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CARDIOVASCULAR ALTERATIONS IN THE ACUTE PHASE OF CHAGAS DISEASE IN SEDENTARY AND TRAINED MICE
Author(s) -
MartinsPinge Marli Cardoso,
Lucchetti Bruno Fernando Cruz,
Ataides Raquel Hiviny,
Zanluqui Nagela Ghabdan,
LovoMartins Maria Izabel,
Tatakihara Vera Lucia,
Michelini Lisete Compagno,
PingeFilho Phileno
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1288.11
Subject(s) - parasitemia , chagas disease , heart rate , medicine , griess test , trypanosoma cruzi , bradycardia , analysis of variance , nitric oxide , blood pressure , cardiology , immunology , biology , nitrite , malaria , parasite hosting , ecology , plasmodium falciparum , world wide web , computer science , nitrate
Trypanosoma cruzi infection causes heart inflammation and fibrosis with changes in the architecture and functionality of the heart. The literature shows that physical activity (PA) of chronic moderate‐intensity acts as a factor of resistance against T. cruzi infection in animals. The parameters of mean arterial pressure, heart rate (HR) and nitric oxide (NO) accompanying the acute phase of infection in animals trained have not been clarified. We aimed to evaluate the cardiovascular parameters, production of NO in the heart and plasma, parasitaemia and mortality in trained mice infected with T. cruzi. This study was approved by our institutional ethic committee (process number: 28105.2014.72). The AF was performed on treadmill for nine weeks. Then, the mice were infected with 5×103 trypomastigotes of T. cruzi (Y strain) or PBS (control). On days 7, 14 and 20 after inoculation (dpi) cardiovascular parameters were evaluated by CODA platform. The parasitemia was performed using the method of Brenner and dosage of NO by the Griess method. Data were analyzed using analysis of variance two‐way ANOVA. We observed resting bradycardia (758bpm vs 703bpm) and a better performance in the performance test (2.2 vs 1.5 km / h) in trained animals (n = 27) compared with sedentary (n = 14). With 20 dpi we found a decrease in HR in infected sedentary animals (SI) (n = 10) compared to the infected trained (TI) (n = 10) (669 vs 765bpm, p<0.05). We observed a decreased production of NO in cardiac tissue in IT compared with IS (6,51 vs 20.73 NO2μM). We observed a decrease in parasitemia in IT compared to IS in the 15 dpi (2,03×10 6 vs 1,17×10 6 parasites / ml) and 17 dpi (3,37×106 vs 1,02×106 parasites / ml). IT animals showed increased survival compared to SI. Exercise training improves cardiovascular parameters during infection by T. cruzi, contributing to the increased survival of infected animals, and NO may participate in this process. Support or Funding Information CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior)