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Effect of Post‐Exercise Ethanol on Signaling Pathways Regulating Mitochondrial Biogenesis
Author(s) -
Engel Amy L.,
Kanhere Arun P.,
Jimenez R. Tomas,
Coleman Denver J.,
Blank Jason M
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1287.8
Subject(s) - mitochondrial biogenesis , soleus muscle , medicine , chemistry , ethanol , endocrinology , creb , saline , skeletal muscle , p38 mitogen activated protein kinases , physical exercise , mitochondrion , mapk/erk pathway , phosphorylation , biology , biochemistry , gene , transcription factor
Ethanol is known to impair protein synthesis in muscle via inhibition of IGF‐1 signaling, but ethanol effects on pathways regulating mitochondrial biogenesis are not as clear. This study focuses on the combined effects of ethanol and exercise on mitochondrial biogenesis in skeletal muscle. Female rats (n=48) were randomly assigned to one of 4 groups: exercise followed by ethanol injection (RunEth), exercise with saline injection (RunSal), non‐exercise with ethanol injection (SitEth), or non‐exercise with saline injection (SitSal). Rats in the exercise groups completed interval running workouts to exhaustion on a treadmill (60 to 90 minutes duration). Immediately after exercise, rats were injected intraperitoneally with ethanol (75 mmols/kg) or an equivalent volume of saline. Three hours after the injection, hindlimb muscles were harvested and frozen in liquid nitrogen. Western blots were used to measure phosphorylation and/or abundance of proteins involved in regulating mitochondrial biogenesis (PGC‐1a, p‐p38 MAPK, p‐CREB) in samples from both soleus and plantaris. In plantaris of saline injected rats, acute exercise increased the abundance of PGC‐1a to 2.5 times the resting level (p=0.002), but the exercise‐induced increase was blocked by ethanol (p=0.89). p‐p38 MAPK levels in plantaris were not altered by running (p=0.16) or ethanol (p=0.81). Neither exercise nor ethanol altered PGC‐1a, p‐p38 MAPK, or p‐CREB in soleus 3 hours after exercise. Analysis of mRNA responses is ongoing.