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Characterization of Respiratory Activity in Spontaneously Breathing Urethane‐Anesthetized 6‐OHDA SN‐lesioned Parkinson's Disease Rat Model
Author(s) -
Solomon Irene C,
Collins William F
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1285.3
Subject(s) - hypercapnia , respiratory system , control of respiration , parkinson's disease , respiratory center , lesion , basal (medicine) , medicine , basal ganglia , breathing , ventilation (architecture) , anesthesia , chemistry , neuroscience , cardiology , biology , central nervous system , pathology , disease , mechanical engineering , insulin , engineering
Respiratory dysfunction in Parkinson's Disease (PD) patients manifests as a variety of altered breathing patterns that are suggested to result from impairment of central respiratory control. To date, only a limited number of studies have used a rat PD model to begin to explore respiratory aspects of PD. Therefore, to better characterize the respiratory phenotype of PD, we have begun a series of experiments in the 6‐hydroxydopamine (6‐OHDA) neurotoxin‐induced unilateral SN lesion rat model. For these experiments, spontaneously breathing urethane‐anesthetized rats are being exposed to room air (basal conditions) and either hypoxic (12% O 2 ) or hypercapnic (7% CO 2 ) gas, and inspiratory‐related (tongue and/or diaphragm), and in some cases expiratory‐related (external oblique), EMG activity are recorded. Our preliminary data indicate that compared to control rats, SN‐lesioned rats (at 1‐week or 1‐month post‐lesion) exhibited (1) significantly higher basal breathing frequencies; (2) a similar increase in frequency, but a variable increase in the magnitude of the amplitude response to hypoxia; and (3) a slightly enhanced increase in breathing frequency and amplitude in response hypercapnia. These preliminary observations suggest that (1) the 6‐OHDA‐lesioned rat/rodent is a viable model for studying respiratory abnormalities in PD and (2) PD may differentially affect hypoxic and hypercapnic ventilatory responses. Support or Funding Information Thomas Hartman Center for Parkinson's Research at Stony Brook University