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Hemodynamics and Premature Ventricular Beats in Conscious Rats with Acute Myocardial Infarction Treated with Pyridostigmine Bromide
Author(s) -
SantosAlmeida Fernanda Machado,
Salgado Helio Cesar,
Fazan Rubens
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.1278.8
Subject(s) - medicine , pyridostigmine , myocardial infarction , hemodynamics , anesthesia , saline , cardiology , heart rate , ventricular pressure , ventricle , left coronary artery , blood pressure , myasthenia gravis
We examined the effect of the acetyl‐cholinesterase inhibitor pyridostigmine bromide (PYR) on hemodynamics and arrhythmias in conscious rats after acute myocardial infarction (MI). Male Wistar rats were anesthetized (ketamine/xylazine) and implanted with subcutaneous electrocardiogram (ECG) electrodes, and catheters into the left femoral artery and vein. At the following day, under inhalatory anesthesia (isoflurane), animals were subjected to coronary artery ligation to induce acute MI in the anterior wall of the left ventricle. Control rats were submitted to fictitious coronary ligation (sham surgery). 20 and 120 min after MI, with the animals already recovered from anesthesia, they were treated with PYR (0.25 mg/kg, iv), or saline. The arterial pressure (AP) and ECG were continuously recorded between the third and fourth hour after acute MI. AP as found similar among groups (92±7, 92±10 and 97±8 mmHg). Heart rate (HR) was markedly higher in non‐treated (saline) infarcted rats (442±15 bpm) as compared to control (non‐infarcted) counterparts (389±10 bpm). PYR significantly reduced HR of infarcted rats (414±9 bpm), but it remained higher than non‐infarcted control rats. In addition, infarcted rats treated with PYR showed lower number of premature ventricular beats (0 to 99 events per hour) as compared to non‐treated group (1 to 169 events per hour). In conclusion, the treatment with PYR, on the first hours after MI, showed a protective effect preserving hemodynamics (bradycardic effect without changing AP), and also reducing the incidence of ventricular arrhythmias. Support or Funding Information FAPESP, CNPq, FAEPA